Research Paper Volume 13, Issue 4 pp 6115—6133

Identification of miRNAs as diagnostic and prognostic markers in hepatocellular carcinoma

Effects of let-7e expression on apoptosis and autophagy in HCC cells. (A) Upregulated let-7e suppressed apoptosis of HepG2 cells, but downregulated let-7e initiated apoptosis in these cells. (B) Upregulated let-7e suppressed apoptosis of Hep3B cells, but downregulated let-7e initiated apoptosis in these cells. (C) WB analysis was used to detect the expression of several key cell apoptotic proteins in let-7e mimic–transfected HepG2 cells and NC mimic–transfected HepG2 cells. (D) Determination of the expression of several key cell apoptotic proteins in let-7e mimic–transfected Hep3B cells and NC mimic–transfected Hep3B cells. (E) Determination of the expression of key autophagy regulators in let-7e mimic–transfected HepG2 cells and NC mimic–transfected HepG2 cells. (F) Determination of the expression of key autophagy regulators in let-7e mimic–transfected Hep3B cells and NC mimic–transfected Hep3B cells (*PPP

Figure 6. Effects of let-7e expression on apoptosis and autophagy in HCC cells. (A) Upregulated let-7e suppressed apoptosis of HepG2 cells, but downregulated let-7e initiated apoptosis in these cells. (B) Upregulated let-7e suppressed apoptosis of Hep3B cells, but downregulated let-7e initiated apoptosis in these cells. (C) WB analysis was used to detect the expression of several key cell apoptotic proteins in let-7e mimic–transfected HepG2 cells and NC mimic–transfected HepG2 cells. (D) Determination of the expression of several key cell apoptotic proteins in let-7e mimic–transfected Hep3B cells and NC mimic–transfected Hep3B cells. (E) Determination of the expression of key autophagy regulators in let-7e mimic–transfected HepG2 cells and NC mimic–transfected HepG2 cells. (F) Determination of the expression of key autophagy regulators in let-7e mimic–transfected Hep3B cells and NC mimic–transfected Hep3B cells (*P<0.05, **P<0.01, ***P<0.001). The NC mimic–transfected cells were as the control group in all experiments.