Figure 2. CircRHOT1 enhances invasion and migration of breast cancer cells. (A) The MDA-MB-231 cells were treated with control shRNA or circRHOT1 shRNA. The expression of circRHOT1 was measured by qPCR in the cells. (B–D) The MDA-MB-231 and T47D cells were treated with control shRNA or circRHOT1 shRNA. (B, C) The cell migration and invasion were analyzed by transwell assays in the cells. (D, E) The migration and invasion were examined by wound healing assays in the cells. The wound healing proportion was shown. Data are presented as mean ± SD. Statistic significant differences were indicated: ** P < 0.01.