Research Paper Volume 13, Issue 4 pp 4881—4894

MiR-141-3p promotes mitochondrial dysfunction in ovariectomy-induced sarcopenia via targeting Fkbp5 and Fibin

miR-141-3p targets Fkbp5 and Fibin. (A) Heatmap of significantly up and down-regulated differentially expressed mRNA in OVX mice. (B) Differentially expressed miRNA in OVX mice. (C) The putative binding site between miR-141-3p and its targets, Fkbp5 and Fibin. (D) The upregulation of miR-141-3p in OVX mice was validated by qRT-PCR. (E, F) The expressions of Fkbp5 and Fibin in mRNA (E) and protein (F) levels. (G, H) A reporter vector containing Fkbp5 or Fibin 3’UTR was transfected along with miR-141-3p mimic (G) or inhibitor (H). The luciferase activity was normalized to renilla luciferase activity. (I, J) Measurement of Fkbp5 or Fibin expressions after exposure miR-141-3p mimic (I) or inhibitor (J). Data are means ± SEM. *PP

Figure 3. miR-141-3p targets Fkbp5 and Fibin. (A) Heatmap of significantly up and down-regulated differentially expressed mRNA in OVX mice. (B) Differentially expressed miRNA in OVX mice. (C) The putative binding site between miR-141-3p and its targets, Fkbp5 and Fibin. (D) The upregulation of miR-141-3p in OVX mice was validated by qRT-PCR. (E, F) The expressions of Fkbp5 and Fibin in mRNA (E) and protein (F) levels. (G, H) A reporter vector containing Fkbp5 or Fibin 3’UTR was transfected along with miR-141-3p mimic (G) or inhibitor (H). The luciferase activity was normalized to renilla luciferase activity. (I, J) Measurement of Fkbp5 or Fibin expressions after exposure miR-141-3p mimic (I) or inhibitor (J). Data are means ± SEM. *P<0.05, **P<0.01; unpaired two-tailed Student’s t-test.