Research Paper Volume 13, Issue 4 pp 4881—4894

MiR-141-3p promotes mitochondrial dysfunction in ovariectomy-induced sarcopenia via targeting Fkbp5 and Fibin

E2 reverses miR-141-3p/Fkbp5 and Fibin signaling. (A) Relative mRNA expressions of estrogen receptor 1 or 2 (ESR1/2) and lipid metabolism associated genes (PLIN2 and FASN). Data are means ± SEM. *PPB) Western blot analysis for PLIN2 and FASN. (C) Relative mRNA expression of OVX-related genes in PA or PA+E2 treated C2C12 myotubes. (D) The miR-141-3p expression in PA or PA+E2 treated C2C12 myotubes. (E, F) Expressions of Fkbp5 and Fibin in mRNA (E) and protein (F) levels from PA or PA+E2 treated C2C12 myotubes. *PPG–I) The effect of miR-141-3p knockdown on PA induced miR-141-3p expression (G), Fkbp5 and Fibin expressions in mRNA and protein levels (H, I, respectively). **P<0.01 vs Inh

Figure 5. E2 reverses miR-141-3p/Fkbp5 and Fibin signaling. (A) Relative mRNA expressions of estrogen receptor 1 or 2 (ESR1/2) and lipid metabolism associated genes (PLIN2 and FASN). Data are means ± SEM. *P<0.05, **P<0.01; unpaired two-tailed Student’s t-test. (B) Western blot analysis for PLIN2 and FASN. (C) Relative mRNA expression of OVX-related genes in PA or PA+E2 treated C2C12 myotubes. (D) The miR-141-3p expression in PA or PA+E2 treated C2C12 myotubes. (E, F) Expressions of Fkbp5 and Fibin in mRNA (E) and protein (F) levels from PA or PA+E2 treated C2C12 myotubes. *P<0.05, **P<0.01 vs PA-treated C2C12 myotubes. (GI) The effect of miR-141-3p knockdown on PA induced miR-141-3p expression (G), Fkbp5 and Fibin expressions in mRNA and protein levels (H, I, respectively). **P<0.01 vs Inh_CTL-treated C2C12 cells. (J) After incubation for 24h with propylpyrazoletriol (PPT), an ESR1 agonist, the expression of miR-141-3p was measured in C2C12 cells. **P<0.01 vs control. Data are means ± SD. one-way ANOVA with post hoc Dunnett’s multiple comparison test. (K) The miR-141-3p/Fkbp5 and Fibin signaling in sarcopenic obesity.