Research Paper Volume 13, Issue 4 pp 4831—4849

An aging and p53 related marker: HOXA5 promoter methylation negatively correlates with mRNA and protein expression in old age

Deep analysis of CpG sites. (A) Validated methylation values of the microarray-detected CpG sites are demonstrated in young and old subjects. Detection of mean DNA methylation was performed by local deep sequencing on bisulfite-converted DNA prepared from monocytes. The associated genes are assigned to the target CpG site, respectively. Error bars denote SEM (n = 20, average age: 23.4 and 79.6 yrs, respectively). (B–D) shows the correlation between the methylation of the target CpG site of DUSP3, HOXA5 and RYR1 and the participant's age. Methylation values of the 8 individuals that were originally applied in the MethylationEPIC BeadChip Microarray are accentuated by red dots. (B) Depicts the correlation between the methylation of the target CpG site of DUSP3 and the participants age showing a very low correlation coefficient (r = -0.0255). (C) Delineates a linear correlation between HOXA5 methylation in the target CpG site of the HOXA5 promoter region and the participants age showing increasing methylation levels with increasing age as reflected in the respective correlation coefficient (r = 0.7437). (D) Depicts the correlation between the methylation of the target CpG site of RYR1 and and the participants age showing a very low correlation coefficient (r = 0.1174).

Figure 3. Deep analysis of CpG sites. (A) Validated methylation values of the microarray-detected CpG sites are demonstrated in young and old subjects. Detection of mean DNA methylation was performed by local deep sequencing on bisulfite-converted DNA prepared from monocytes. The associated genes are assigned to the target CpG site, respectively. Error bars denote SEM (n = 20, average age: 23.4 and 79.6 yrs, respectively). (BD) shows the correlation between the methylation of the target CpG site of DUSP3, HOXA5 and RYR1 and the participant's age. Methylation values of the 8 individuals that were originally applied in the MethylationEPIC BeadChip Microarray are accentuated by red dots. (B) Depicts the correlation between the methylation of the target CpG site of DUSP3 and the participants age showing a very low correlation coefficient (r = -0.0255). (C) Delineates a linear correlation between HOXA5 methylation in the target CpG site of the HOXA5 promoter region and the participants age showing increasing methylation levels with increasing age as reflected in the respective correlation coefficient (r = 0.7437). (D) Depicts the correlation between the methylation of the target CpG site of RYR1 and and the participants age showing a very low correlation coefficient (r = 0.1174).