Research Paper Volume 13, Issue 4 pp 6194—6204

USP30 protects against oxygen-glucose deprivation/reperfusion induced mitochondrial fragmentation and ubiquitination and degradation of MFN2

Ubiquitination of MFN2 in the early stage of reperfusion after 4 h OGD. (A) After immunoprecipitation of MFN2, western blot using an antibody against ubiquitin was performed to examine the ubiquitination of MFN2 after 45 min reperfusion following 4 h OGD in SK-N-BE(2) cells. (B) Quantitation (Mean ± SEM) of ubiquitinated MFN2 from three independent experiments. (C) Quantitation (Mean ± SEM) of total MFN2 from three independent experiments. There was a significant increase in the ubiquitination of MFN2 during early reperfusion after 4 h OGD.

Figure 2. Ubiquitination of MFN2 in the early stage of reperfusion after 4 h OGD. (A) After immunoprecipitation of MFN2, western blot using an antibody against ubiquitin was performed to examine the ubiquitination of MFN2 after 45 min reperfusion following 4 h OGD in SK-N-BE(2) cells. (B) Quantitation (Mean ± SEM) of ubiquitinated MFN2 from three independent experiments. (C) Quantitation (Mean ± SEM) of total MFN2 from three independent experiments. There was a significant increase in the ubiquitination of MFN2 during early reperfusion after 4 h OGD.