Research Paper Volume 13, Issue 4 pp 6194—6204

USP30 protects against oxygen-glucose deprivation/reperfusion induced mitochondrial fragmentation and ubiquitination and degradation of MFN2

Expression of mitochondrial outer membrane protein MFN2 and mitochondrial inner membrane protein TIM23 in the early stage of reperfusion after 4 h OGD. (A) Western blot was performed to examine the protein level of MFN2 and TIM23 in the early stage of reperfusion following 4 h OGD in SK-N-BE(2) cells. Tubulin was as a loading control. (B) Quantitation (Mean ± SEM) of A from three independent experiments. The expression of MFN2 was reduced in a time-dependent manner during early reperfusion after OGD, whereas the expression of TIM23 was unchanged.

Figure 3. Expression of mitochondrial outer membrane protein MFN2 and mitochondrial inner membrane protein TIM23 in the early stage of reperfusion after 4 h OGD. (A) Western blot was performed to examine the protein level of MFN2 and TIM23 in the early stage of reperfusion following 4 h OGD in SK-N-BE(2) cells. Tubulin was as a loading control. (B) Quantitation (Mean ± SEM) of A from three independent experiments. The expression of MFN2 was reduced in a time-dependent manner during early reperfusion after OGD, whereas the expression of TIM23 was unchanged.