Figure 3. Expression of mitochondrial outer membrane protein MFN2 and mitochondrial inner membrane protein TIM23 in the early stage of reperfusion after 4 h OGD. (A) Western blot was performed to examine the protein level of MFN2 and TIM23 in the early stage of reperfusion following 4 h OGD in SK-N-BE(2) cells. Tubulin was as a loading control. (B) Quantitation (Mean ± SEM) of A from three independent experiments. The expression of MFN2 was reduced in a time-dependent manner during early reperfusion after OGD, whereas the expression of TIM23 was unchanged.