Research Paper Volume 13, Issue 4 pp 6194—6204

USP30 protects against oxygen-glucose deprivation/reperfusion induced mitochondrial fragmentation and ubiquitination and degradation of MFN2

Effect of USP30 overexpression on mitochondrial morphology in SK-N-BE(2) cells exposed to OGDR. SK-N-BE(2) cells were transfected with indicated plasmids. After transfection 36 h, SK-N-BE(2) cells were treated with 4 h OGD plus 4 h reperfusion. (A) Digital photomicrograph under fluorescent illumination showed the morphology of mitochondria by mito-GFP. Mitochondrial fragmentation was evident in SK-N-BE(2) cells after 4 h reperfusion following 4 h OGD. However, USP30(200ng or 500ng) transfection significantly increased the number of SK-N-BE(2) cells with typical tubular and long mitochondria without fragmentation in a dose-dependent manner. (B) Quantitation (Mean ± SEM) of A from three independent experiments. Transfection with USP30(200ng or 500ng) protected against OGDR-induced mitochondrial fragmentation in a dose-dependent manner. OE: over expression.

Figure 5. Effect of USP30 overexpression on mitochondrial morphology in SK-N-BE(2) cells exposed to OGDR. SK-N-BE(2) cells were transfected with indicated plasmids. After transfection 36 h, SK-N-BE(2) cells were treated with 4 h OGD plus 4 h reperfusion. (A) Digital photomicrograph under fluorescent illumination showed the morphology of mitochondria by mito-GFP. Mitochondrial fragmentation was evident in SK-N-BE(2) cells after 4 h reperfusion following 4 h OGD. However, USP30(200ng or 500ng) transfection significantly increased the number of SK-N-BE(2) cells with typical tubular and long mitochondria without fragmentation in a dose-dependent manner. (B) Quantitation (Mean ± SEM) of A from three independent experiments. Transfection with USP30(200ng or 500ng) protected against OGDR-induced mitochondrial fragmentation in a dose-dependent manner. OE: over expression.