Research Paper Volume 13, Issue 6 pp 8214—8227

Bromodomain-containing protein 4 silencing by microRNA-765 produces anti-ovarian cancer cell activity

Ectopic overexpression of miR-765 induces significant anti-ovarian cancer cell activity. Primary ovarian cancer cells (pOC-1 and pOC-2) (A–J) or established cell lines (CaOV3 and SKOV3) (G–J) were transduced with the lentiviral construct encoding pre-miR-765 sequence (lv-pre-miR-765) or scramble non-sense miRNA (lv-miRC). After selection by puromycin stable cells were established. Cells were further cultured for applied time periods, cell growth (A), cell viability (CCK-8 OD, B, H), colony formation (C) and proliferation (by counting EdU-positive nuclei ratio, D, G), as well as cell migration (“Transwell” assays, E, I) and invasion (“Matrigel Transwell” assays, F, J) were tested, and results quantified. “Pare” stands for the parental control cells. For each assay, n=5 (five replicate well/dishes). Data were presented as mean ± standard deviation (SD). * p D–F).

Figure 2. Ectopic overexpression of miR-765 induces significant anti-ovarian cancer cell activity. Primary ovarian cancer cells (pOC-1 and pOC-2) (AJ) or established cell lines (CaOV3 and SKOV3) (GJ) were transduced with the lentiviral construct encoding pre-miR-765 sequence (lv-pre-miR-765) or scramble non-sense miRNA (lv-miRC). After selection by puromycin stable cells were established. Cells were further cultured for applied time periods, cell growth (A), cell viability (CCK-8 OD, B, H), colony formation (C) and proliferation (by counting EdU-positive nuclei ratio, D, G), as well as cell migration (“Transwell” assays, E, I) and invasion (“Matrigel Transwell” assays, F, J) were tested, and results quantified. “Pare” stands for the parental control cells. For each assay, n=5 (five replicate well/dishes). Data were presented as mean ± standard deviation (SD). * p < 0.05 vs. “lv-miRC” cells. Experiments in this figure were repeated five times with similar results obtained. Scale bar=100 μm (DF).