Research Paper Volume 13, Issue 6 pp 8214—8227

Bromodomain-containing protein 4 silencing by microRNA-765 produces anti-ovarian cancer cell activity

Ectopic overexpression of miR-765 induces apoptosis activation in ovarian cancer cells. Primary ovarian cancer cells (pOC-1 and pOC-2) (A–F) or established cell lines (CaOV3 and SKOV3) (E–G) were transduced with the lentiviral construct encoding pre-miR-765 sequence (lv-pre-miR-765) or scramble non-sense miRNA (lv-miRC). After selection by puromycin stable cells were established. Cells were further cultured for applied time periods, caspase-3 activation (A, E). Histone-bound DNA contents (ELISA assays, B) and mitochondrial depolarization (by measuring JC-1 green monomer intensity, C, F) were tested. Cell apoptosis was tested by TUNEL-nuclei staining assay (D, G). “Pare” stands for the parental control cells. For each assay, n=5 (five replicate well/dishes). Data were presented as mean ± standard deviation (SD). * p C, D).

Figure 3. Ectopic overexpression of miR-765 induces apoptosis activation in ovarian cancer cells. Primary ovarian cancer cells (pOC-1 and pOC-2) (AF) or established cell lines (CaOV3 and SKOV3) (EG) were transduced with the lentiviral construct encoding pre-miR-765 sequence (lv-pre-miR-765) or scramble non-sense miRNA (lv-miRC). After selection by puromycin stable cells were established. Cells were further cultured for applied time periods, caspase-3 activation (A, E). Histone-bound DNA contents (ELISA assays, B) and mitochondrial depolarization (by measuring JC-1 green monomer intensity, C, F) were tested. Cell apoptosis was tested by TUNEL-nuclei staining assay (D, G). “Pare” stands for the parental control cells. For each assay, n=5 (five replicate well/dishes). Data were presented as mean ± standard deviation (SD). * p < 0.05 vs. “lv-miRC” cells. Experiments in this figure were repeated five times with similar results obtained. Scale bar=100 μm (C, D).