Figure 6. Effects of calycosin on xenograft tumor growth and the expression levels of RP11-65M17.3, ERα and BRIP1 in tumor tissue. (A–C) Nude mice bearing MCF-7-xenografted were treated for 20 days with calycosin (0 or 8 mg/kg) and 20 μg/kg E2. The tumor volume and tumor weight of the MCF-7 xenografts were measured (n = 6). (D) The tumor samples were subjected to IHC staining for BRIP1. (E–G) The mRNA expression levels of RP11-65M17.3, ERα and BRIP1 in the tumor tissues were determined using qRT-PCR, and β-actin served as the control. Representative data from three independent experiments are shown. *p < 0.05 vs. control; #p < 0.05 vs. 8 mg/kg calycosin.