Research Paper Volume 13, Issue 6 pp 8380—8395

Extracellular HMGB1 promotes CD44 expression in hepatocellular carcinoma via regulating miR-21

HMGB1 is positively associated with CD44 in HCC. (A) Representative images show different intensities of staining CD44 and cytoplasmic HMGB1 in HCC samples. Scale bars, 100um. (B) HCC Patients with high expression of cytoplasmic HMGB1 are characterized by high expression of CD44 through analyzing IHC score. n=68. (C) Positive correlation of CD44 and HMGB1 in HCC samples by analyzing the mRNA levels. n=50. (D, E) Different expression of CD44, HMGB1 and supernatant HMGB1 (HMGB1-sup) are measured in five HCC cell lines via immunoblot analysis and results are quantified. HMGB1-sup rather than HMGB1 is positively correlated with CD44. Data are means ± SEM, * means p

Figure 1. HMGB1 is positively associated with CD44 in HCC. (A) Representative images show different intensities of staining CD44 and cytoplasmic HMGB1 in HCC samples. Scale bars, 100um. (B) HCC Patients with high expression of cytoplasmic HMGB1 are characterized by high expression of CD44 through analyzing IHC score. n=68. (C) Positive correlation of CD44 and HMGB1 in HCC samples by analyzing the mRNA levels. n=50. (D, E) Different expression of CD44, HMGB1 and supernatant HMGB1 (HMGB1-sup) are measured in five HCC cell lines via immunoblot analysis and results are quantified. HMGB1-sup rather than HMGB1 is positively correlated with CD44. Data are means ± SEM, * means p<0.05, ** means p<0.01, *** means p<0.001 by unpaired student T test.