Research Paper Volume 13, Issue 6 pp 8454—8466

Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids facilitates the repair of cardiotoxin-induced skeletal muscle injury in fat-1 mice

High endogenous n-3 PUFA levels reduce inflammation response to cardiotoxin-induced muscle injury in fat-1 mice. (A) Representative images show Masson's trichrome staining of the gastrocnemius muscle sections from fat-1 and wild-type mice on days 7 and 14 days after CTX-induced injury. Scale bar = 100 μm. (B) Quantitative data for the fibrosis area. (C, D) Representative immunofluorescence images and quantitative analysis show α-SMA protein expression in the gastrocnemius muscle tissues from fat-1 and wild-type mice on day 7 after CTX-induced injury. Scale bar = 50 μm. Data were expressed as the mean ± SD (n = 4-5). NS, non-significant; * P P P

Figure 3. High endogenous n-3 PUFA levels reduce inflammation response to cardiotoxin-induced muscle injury in fat-1 mice. (A) Representative images show Masson's trichrome staining of the gastrocnemius muscle sections from fat-1 and wild-type mice on days 7 and 14 days after CTX-induced injury. Scale bar = 100 μm. (B) Quantitative data for the fibrosis area. (C, D) Representative immunofluorescence images and quantitative analysis show α-SMA protein expression in the gastrocnemius muscle tissues from fat-1 and wild-type mice on day 7 after CTX-induced injury. Scale bar = 50 μm. Data were expressed as the mean ± SD (n = 4-5). NS, non-significant; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT.