Research Paper Volume 13, Issue 5 pp 7608—7626
MiR-452-5p promotes colorectal cancer progression by regulating an ERK/MAPK positive feedback loop
Figure 2. MiR-452-5p promotes malignant biological behaviors of CRC cells. (A) The expression levels of miR-452-5p in colorectal cancer cell lines and human normal intestinal epithelial cell line FHC. (B) Instantaneous transfection efficiency verification of miR-452-5p. (C) Effects of overexpression and knockdown of miR-452-5p on the proliferation of CRC cells: CCK8 assay. (D) Effects of overexpression and knockdown of miR-452-5p on the proliferation of CRC cells: panel cloning assay. (E) Effects of overexpression of miR-452-5p on subcutaneous tumor formation in nude mice. (F) Effects of overexpression and knockdown of miR-452-5p on CRC cell cycle: cell cycle detection by flow cytometry. (G) Cell cycle related-proteins detected by western blotting.*p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant.