Figure 4. MiR-452-5p directly targets PKN2 and DUSP6. (A) Bioinformatics prediction of potential miR-452-5p target genes by 4 miRNA databases. (B) QRT-PCR results of candidate target genes. (C) The expression of PKN2 and DUSP6 after overexpression and knockdown of miR-452-5p detected by western blotting. (D) The expression of PKN2 and DUSP6 after overexpression and knockdown of miR-452-5p detected by qRT-PCR. (E) Luciferase reporter gene assay: binding sites and mutation sites of PKN2 and DUSP6 were shown on the left, luciferase reporter gene results were shown on the right. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant.