Figure 6. C-jun regulates the expression of miR-452-5p by promoting its transcription. (A) Heatmap analysis showed the MAPK signaling pathway genes as identified by sequencing. (B) Western blotting results of ERK/MAPK signaling pathway. (C) The expression of miR-452-5p after overexpression of c-Jun detected by qRT-PCR. (D) Luciferase reporter gene assay verified the direct transcriptional regulation effect of c-Jun on miR-452-5p. (E) A hypothetical model illustrating that miR-452-5p promotes the malignant behaviors of CRC through the miR-452-5p—PKN2/DUSP6—c-Jun positive feedback loop.*p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant.