Priority Research Paper Volume 13, Issue 4 pp 4747—4777

Insights into epithelial cell senescence from transcriptome and secretome analysis of human oral keratinocytes

Unbiased, global mass spectrometry of CM and EVs. (A) Mass spectrometry of proteins isolated from CM by UC shows enrichment for exosomal markers. Mass spectrometry results are expressed as NSAF per 106 cells (spectral abundance factor normalized for protein size and cell count). (B) Overrepresentation analysis of proteins significantly increased in CM with senescence. Y-axis shows enrichment ratio. (C) Overrepresentation analysis of proteins significantly increased in EV pellet with senescence. Y-axis shows enrichment ratio. (B, C, p

Figure 4. Unbiased, global mass spectrometry of CM and EVs. (A) Mass spectrometry of proteins isolated from CM by UC shows enrichment for exosomal markers. Mass spectrometry results are expressed as NSAF per 106 cells (spectral abundance factor normalized for protein size and cell count). (B) Overrepresentation analysis of proteins significantly increased in CM with senescence. Y-axis shows enrichment ratio. (C) Overrepresentation analysis of proteins significantly increased in EV pellet with senescence. Y-axis shows enrichment ratio. (B, C, p < 0.01 comparing late to early and mid to early).