Figure 4. Nicotine activates the pyroptosis pathway in BMDMs by upregulating ROS production. (A, B) Western blot was used to detect the expression of TXNIP, NLRP3, ASC, caspase-1, and GSDMD. The average ratios were calculated based on gray intensity analysis (*P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001, n = 4). (C) IL-1β and IL18 in the supernatant of BMDMs treated with or without nicotine were measured by ELISA (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n=5).