Research Paper Volume 13, Issue 6 pp 8643—8664

Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathy

The expression of FSTL1, TGFβ, CTGF, VEGF, FN, COL1 and α-SMA proteins in FSTL1 overexpression group and FSTL1 knockdown group. (A) shows western blots representatives of FSTL1, TGFβ, CTGF, VEGF, FN, COL1, α-SMA and β-actin in HUVECs. (B–H) shows the ratio of FSTL1, TGFβ, CTGF, VEGF, FN, COL1 and α-SMA to β-actin in the blank control group (N), negative control group (NC), FSTL1 overexpression group (Overexpression) and FSTL1 siRNA group (Knockdown) respectively. The western blots figures were quantitative analyzed by ImageJ. FSTL1, TGFβ, VEGF, FN and α-SMA proteins were all upregulated in FSTL1 overexpression group compared with negative control group, and the expression of FSTL1, TGFβ, CTGF, VEGF, FN, COL1, and α-SMA proteins were all downregulated in FSTL1 knockdown group compared with negative control group (N: blank control group, NC: negative control group, Overexpression: FSTL1 overexpression group, Knockdown: FSTL1 siRNA group). Data are expressed as mean ± SEM. * P

Figure 11. The expression of FSTL1, TGFβ, CTGF, VEGF, FN, COL1 and α-SMA proteins in FSTL1 overexpression group and FSTL1 knockdown group. (A) shows western blots representatives of FSTL1, TGFβ, CTGF, VEGF, FN, COL1, α-SMA and β-actin in HUVECs. (BH) shows the ratio of FSTL1, TGFβ, CTGF, VEGF, FN, COL1 and α-SMA to β-actin in the blank control group (N), negative control group (NC), FSTL1 overexpression group (Overexpression) and FSTL1 siRNA group (Knockdown) respectively. The western blots figures were quantitative analyzed by ImageJ. FSTL1, TGFβ, VEGF, FN and α-SMA proteins were all upregulated in FSTL1 overexpression group compared with negative control group, and the expression of FSTL1, TGFβ, CTGF, VEGF, FN, COL1, and α-SMA proteins were all downregulated in FSTL1 knockdown group compared with negative control group (N: blank control group, NC: negative control group, Overexpression: FSTL1 overexpression group, Knockdown: FSTL1 siRNA group). Data are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.