Research Paper Volume 13, Issue 6 pp 8665—8687

MicroRNA-95-3p serves as a contributor to cisplatin resistance in human gastric cancer cells by targeting EMP1/PI3K/AKT signaling

Down-regulation of miR-95-3p leads to decreased cell viability, weakened invasion and migration, and enhanced cell apoptosis. (A) Treatment with miR-95-3p inhibitor led to a decrease in expression of miR-95-3p. (B) MTT assay demonstrated that lower miR-95-3p levels led to weakened cell survival rate of DDP-resistant SGC7901. (C) BrdU assay results indicated that down-regulation of miR-95-3p reduced cell viability of DDP-resistant SGC7901. (D) Cellular apoptosis rate of DDP-resistant SGC7901. (E, F) Transwell assay results demonstrated lower cell invasion and metastasis of DDP-resistant SGC7901 upon down-regulation of miR-95-3p. (G) Wound healing assay suggested that down-regulation of miR-95-3p led to decreased invasion in DDP-resistant SGC7901. (H) Western blot assay helped examine the down-stream regulatory proteins of miR-95-3p. ***p

Figure 3. Down-regulation of miR-95-3p leads to decreased cell viability, weakened invasion and migration, and enhanced cell apoptosis. (A) Treatment with miR-95-3p inhibitor led to a decrease in expression of miR-95-3p. (B) MTT assay demonstrated that lower miR-95-3p levels led to weakened cell survival rate of DDP-resistant SGC7901. (C) BrdU assay results indicated that down-regulation of miR-95-3p reduced cell viability of DDP-resistant SGC7901. (D) Cellular apoptosis rate of DDP-resistant SGC7901. (E, F) Transwell assay results demonstrated lower cell invasion and metastasis of DDP-resistant SGC7901 upon down-regulation of miR-95-3p. (G) Wound healing assay suggested that down-regulation of miR-95-3p led to decreased invasion in DDP-resistant SGC7901. (H) Western blot assay helped examine the down-stream regulatory proteins of miR-95-3p. ***p<0.001, **p<0.01 compared to inhibitor NC.