Research Paper Volume 13, Issue 6 pp 8797—8816

Functional predication of differentially expressed circRNAs/lncRNAs in the prefrontal cortex of Nrf2-knockout mice

Validation of the DEcircRNAs and DElncRNAs. The levels of six DEcircRNAs and eight DElncRNAs were validated in prefrontal cortex samples from Nrf2 (-/-) and Nrf2 (+/+) mice using qRT-PCR and normalized to the internal reference gene (β-actin). (A) The qRT-PCR results for five of the verified circRNAs (all but mmu

Figure 2. Validation of the DEcircRNAs and DElncRNAs. The levels of six DEcircRNAs and eight DElncRNAs were validated in prefrontal cortex samples from Nrf2 (-/-) and Nrf2 (+/+) mice using qRT-PCR and normalized to the internal reference gene (β-actin). (A) The qRT-PCR results for five of the verified circRNAs (all but mmu_circ_0003404) were consistent with the microarray results. (B) The qRT-PCR results for all eight verified lncRNAs were consistent with the microarray results. All experiments were replicated three times. The deep red column indicates the FCs of the DEcircRNAs/DElncRNAs determined through microarray analyses; the blue column indicates the expression FCs of the DEcircRNAs/DElncRNAs determined through qRT-PCR experiments. The presented values are the means ± standard deviations. Values > 1 indicate up-regulated DEcircRNAs/DElncRNAs, and values < 1 indicate down-regulated DEcircRNAs/DElncRNAs. *p < 0.05, **p < 0.01.