Research Paper Volume 13, Issue 7 pp 9566—9581

miR-20b and miR-125a promote tumorigenesis in radioresistant esophageal carcinoma cells

miR-20b promotes cell proliferation, migration invasion, the EMT process and inhibits apoptosis. KYSE-150 and KYSE-150R cells were transfected with miR-20b mimic or miR-20b inhibitor or their corresponding negative controls. (A) The cell proliferation assay was performed at the indicated time points. (B) Representative micrographs of cell migration assays (left) and the quantification (right). (C) Representative micrographs of cell invasion assays (left) and the quantification (right). (D) Representative micrographs of cell apoptosis assays (left) and the quantification (right). (E) Western blot analysis revealed that the E-cadherin expression level was decreased, while N-cadherin and Vimentin expression levels were elevated in cells transfected with miR-20b mimic. Data are shown as mean ± SD from three independent experiments. *P

Figure 2. miR-20b promotes cell proliferation, migration invasion, the EMT process and inhibits apoptosis. KYSE-150 and KYSE-150R cells were transfected with miR-20b mimic or miR-20b inhibitor or their corresponding negative controls. (A) The cell proliferation assay was performed at the indicated time points. (B) Representative micrographs of cell migration assays (left) and the quantification (right). (C) Representative micrographs of cell invasion assays (left) and the quantification (right). (D) Representative micrographs of cell apoptosis assays (left) and the quantification (right). (E) Western blot analysis revealed that the E-cadherin expression level was decreased, while N-cadherin and Vimentin expression levels were elevated in cells transfected with miR-20b mimic. Data are shown as mean ± SD from three independent experiments. *P<0.05 by Student’s t-test.