Research Paper Volume 13, Issue 7 pp 9566—9581

miR-20b and miR-125a promote tumorigenesis in radioresistant esophageal carcinoma cells

The effects of miR-20b and PTEN on KYSE-150 and KYSE-150R cells. KYSE-150 and KYSE-150R cells were transfected with miR-con or miR-20b mimic, or co-transfected with PTEN and miR-con/miR-20b mimic. (A) The cell proliferation assay was performed at the indicated time points. (B) Representative micrographs of cell migration assays (left) and the quantification (right). (C) Representative micrographs of cell invasion assays (left) and the quantification (right). (D) Representative micrographs of cell apoptosis assays (left) and the quantification (right). (E) Western blot analysis revealed that transfection of PTEN inhibited the EMT process and co-transfection of miR-20b mimic and PTEN promoted the EMT process. Data are shown as mean ± SD from three independent experiments. *P

Figure 5. The effects of miR-20b and PTEN on KYSE-150 and KYSE-150R cells. KYSE-150 and KYSE-150R cells were transfected with miR-con or miR-20b mimic, or co-transfected with PTEN and miR-con/miR-20b mimic. (A) The cell proliferation assay was performed at the indicated time points. (B) Representative micrographs of cell migration assays (left) and the quantification (right). (C) Representative micrographs of cell invasion assays (left) and the quantification (right). (D) Representative micrographs of cell apoptosis assays (left) and the quantification (right). (E) Western blot analysis revealed that transfection of PTEN inhibited the EMT process and co-transfection of miR-20b mimic and PTEN promoted the EMT process. Data are shown as mean ± SD from three independent experiments. *P<0.05 by Student’s t-test.