Priority Research Paper Volume 13, Issue 4 pp 4778—4793

A Wnt5a-Cdc42 axis controls aging and rejuvenation of hair-follicle stem cells

In vivo treatment with CASIN induces onset of anagen in aged mice. (A) Experimental setup (B) Back skin of representative aged mice and aged mice treated i.p with CASIN, pictures were taken weekly (C) H&E staining of mouse back skin sections (longitudinal) from young, aged animals and the anagen area of skin from aged animals after CASIN treatment (D) Quantification of the size of the skin area with anagen in D-50, old and old CASIN treated animals N≥6 (E) Duration of telogen in young, old and old CASIN treated, N≥6, *P

Figure 4. In vivo treatment with CASIN induces onset of anagen in aged mice. (A) Experimental setup (B) Back skin of representative aged mice and aged mice treated i.p with CASIN, pictures were taken weekly (C) H&E staining of mouse back skin sections (longitudinal) from young, aged animals and the anagen area of skin from aged animals after CASIN treatment (D) Quantification of the size of the skin area with anagen in D-50, old and old CASIN treated animals N≥6 (E) Duration of telogen in young, old and old CASIN treated, N≥6, *P<.05, **P<.01, ***P<.001 (paired student’s t test). Error bars represent s.e.m.