Research Paper Volume 13, Issue 6 pp 8849—8864

HDAC11 inhibition disrupts porcine oocyte meiosis via regulating α-tubulin acetylation and histone modifications

Effects of different doses of JB-3-22 on the porcine oocyte maturation. (A) mRNA expression of HDAC11 in porcine oocytes. GAPDH was used to normalize expression levels. The level of HDAC11 expression in GV oocytes was used as a calibrator (expression set to 1). (B) IF staining for HDAC11 protein in GV, MI and MII porcine oocytes. NC, negative control, MII oocyte stained with secondary antibody but not with primary antibody. Scale bar, 100 μm. (C) The results of Western blotting confirmed the expression of HDAC11 protein in porcine oocytes. (D) The rate of first polar extrusion (PBE) was recorded in control and different concentrations groups treated with JB-3-22 (5 μM, 10 μM, 15 μM, 20 μM) after culture for 44 h in vitro. (E) Typical image of oocyte maturation progression in control and JB-3-22 exposed oocytes. Cumulus cell expansion of oocyte complexes (COCs) and the PBE of denuded oocytes (DOs). Scale bar, 500 μm, 200 μm and 100 μm. The results represent the mean ± standard deviation of three independent experiments. * P

Figure 1. Effects of different doses of JB-3-22 on the porcine oocyte maturation. (A) mRNA expression of HDAC11 in porcine oocytes. GAPDH was used to normalize expression levels. The level of HDAC11 expression in GV oocytes was used as a calibrator (expression set to 1). (B) IF staining for HDAC11 protein in GV, MI and MII porcine oocytes. NC, negative control, MII oocyte stained with secondary antibody but not with primary antibody. Scale bar, 100 μm. (C) The results of Western blotting confirmed the expression of HDAC11 protein in porcine oocytes. (D) The rate of first polar extrusion (PBE) was recorded in control and different concentrations groups treated with JB-3-22 (5 μM, 10 μM, 15 μM, 20 μM) after culture for 44 h in vitro. (E) Typical image of oocyte maturation progression in control and JB-3-22 exposed oocytes. Cumulus cell expansion of oocyte complexes (COCs) and the PBE of denuded oocytes (DOs). Scale bar, 500 μm, 200 μm and 100 μm. The results represent the mean ± standard deviation of three independent experiments. * P < 0.05, and ** P < 0.01.