Research Paper Volume 13, Issue 6 pp 8849—8864

HDAC11 inhibition disrupts porcine oocyte meiosis via regulating α-tubulin acetylation and histone modifications

JB-3-22 treatment elevated the acetylation level of α-Tubulin in porcine oocytes. (A) Images of acetylated α-tubulin in control and JB-3-22-treated oocytes. Scale bar, 5 μm. (B) The fluorescence intensity of α-tubulin acetylation in control and JB-3-22 treated oocytes. The results represent the mean ± standard deviation of three independent experiments. ** P C) The results of Western blotting confirmed the protein levels of acetylated tubulin in control and JB-3-22-treated oocytes.

Figure 3. JB-3-22 treatment elevated the acetylation level of α-Tubulin in porcine oocytes. (A) Images of acetylated α-tubulin in control and JB-3-22-treated oocytes. Scale bar, 5 μm. (B) The fluorescence intensity of α-tubulin acetylation in control and JB-3-22 treated oocytes. The results represent the mean ± standard deviation of three independent experiments. ** P < 0.01. (C) The results of Western blotting confirmed the protein levels of acetylated tubulin in control and JB-3-22-treated oocytes.