Figure 8. JB-3-22 promoted the apoptosis of porcine oocytes and disrupted the subsequent embryonic development of porcine oocytes after parthenogenetic activation. (A) The rates of different development stage embryos were recorded in control and JB-3-22 treated oocytes after parthenogenetic activation. (B) Images of apoptotic cells in blastocysts in control and JB-3-22 treated group respectively. (C) The statistics of total cell number in per blastocyst. (D) Images of H4K16 acetylation at different stages of parthenogenetic embryo development in control and JB-3-22 group. (E) The fluorescence intensity of H4K16 acetylation. The results represent the mean ± standard deviation of three independent experiments. * P < 0.05 and ** P < 0.01. Scale bar, 100 μm.