Research Paper Volume 13, Issue 7 pp 9592—9612

Circular DDX10 is associated with ovarian function and assisted reproductive technology outcomes through modulating the proliferation and steroidogenesis of granulosa cells

Effect of circDDX10 on apoptosis and viability of granulosa cells (GCs). (A) Expression levels of apoptosis-related genes. Mock was a negative control group; (B, C) Expression level of apoptosis-related proteins (200×, bar = 100 μm). (D, E) Cell apoptosis was determined by TUNEL. (F) CCK-8 kit was used to detect the cell viability of each group in vitro for 24 h, 48 h, 72 h and 96 h, respectively. NC was a siRNA negative control and EV was an empty vector control group. Each set of experiments was repeated for three times. *, P P

Figure 4. Effect of circDDX10 on apoptosis and viability of granulosa cells (GCs). (A) Expression levels of apoptosis-related genes. Mock was a negative control group; (B, C) Expression level of apoptosis-related proteins (200×, bar = 100 μm). (D, E) Cell apoptosis was determined by TUNEL. (F) CCK-8 kit was used to detect the cell viability of each group in vitro for 24 h, 48 h, 72 h and 96 h, respectively. NC was a siRNA negative control and EV was an empty vector control group. Each set of experiments was repeated for three times. *, P < 0.05; **, P < 0.01.