Figure 4. Effect of circDDX10 on apoptosis and viability of granulosa cells (GCs). (A) Expression levels of apoptosis-related genes. Mock was a negative control group; (B, C) Expression level of apoptosis-related proteins (200×, bar = 100 μm). (D, E) Cell apoptosis was determined by TUNEL. (F) CCK-8 kit was used to detect the cell viability of each group in vitro for 24 h, 48 h, 72 h and 96 h, respectively. NC was a siRNA negative control and EV was an empty vector control group. Each set of experiments was repeated for three times. *, P < 0.05; **, P < 0.01.