Figure 2. Characterization of EVs derived from RCC cells. (A) Representative EVs were analyzed via electron microscopy, and expression of the EV markers CD9 and TSG101 was assessed via Western blotting. (B) A NanoSight analysis was used to measure EV particle size distributions. The average particle diameters ranged 85.8–201.8 nm and 83.7–201.6 nm for EVs derived from 786-O and Caki-1 cells, respectively.