Research Paper Volume 13, Issue 7 pp 9613—9626

Hypoxia-induced microRNA-155 overexpression in extracellular vesicles promotes renal cell carcinoma progression by targeting FOXO3

Hypoxia impacts miR-155 expression in RCC cell-derived EVs. (A) Hypoxia alters EV protein contents. (B, C) The impact of hypoxia on miR-155 expression in EVs (B) and cells (C). Hypoxia significantly increased protein contents in EVs. (D) miR-155 encapsulation within EVs was confirmed using RNase A and Triton X-100. The results confirmed that miR-155 is present primarily within EVs. This experiment was conducted using three distinct biological replicates. **P ***P

Figure 3. Hypoxia impacts miR-155 expression in RCC cell-derived EVs. (A) Hypoxia alters EV protein contents. (B, C) The impact of hypoxia on miR-155 expression in EVs (B) and cells (C). Hypoxia significantly increased protein contents in EVs. (D) miR-155 encapsulation within EVs was confirmed using RNase A and Triton X-100. The results confirmed that miR-155 is present primarily within EVs. This experiment was conducted using three distinct biological replicates. **P < 0.01, and ***P < 0.001.