Research Paper Volume 13, Issue 7 pp 9646—9664

BMP5 silencing inhibits chondrocyte senescence and apoptosis as well as osteoarthritis progression in mice

Bmp5 is overexpressed in knee articular cartilage tissues of OA patients. (A) Representative images show Safranin O-Fast Green staining and BMP5 immunohistochemical staining in the knee articular cartilage tissues from healthy subjects and OA patients (n=6 each). Scale bar: 10 μm. (B) OARSI scores based on the histochemical staining and corresponding BMP5 immunohistochemical staining of human knee articular cartilage tissue sections from healthy and osteoarthritis patients. (C) Representative western blots show BMP5 protein expression in the knee articular cartilage samples from healthy individuals and OA patients. (D) Representative images show Safranin O-Fast Green staining and BMP5 immunohistochemical staining in the knee articular cartilage sections from sham, DMM-2W and DMM-4W model mice (n=5 each). Scale bars: 10 μm (first line) and 5 μm (others). (E) Representative western blots show the levels of BMP5 protein in the knee articular cartilage sections from sham, DMM-2W and DMM-4W model mice. (F) ELISA assay results show joint fluid BMP5 levels in the healthy and OA patients. (G) BMP5 immunohistochemical staining scores and OARSI scores of the DMM-2W, DMM-4W, and sham model mice are shown. All data are represented as means ± SD.

Figure 1. Bmp5 is overexpressed in knee articular cartilage tissues of OA patients. (A) Representative images show Safranin O-Fast Green staining and BMP5 immunohistochemical staining in the knee articular cartilage tissues from healthy subjects and OA patients (n=6 each). Scale bar: 10 μm. (B) OARSI scores based on the histochemical staining and corresponding BMP5 immunohistochemical staining of human knee articular cartilage tissue sections from healthy and osteoarthritis patients. (C) Representative western blots show BMP5 protein expression in the knee articular cartilage samples from healthy individuals and OA patients. (D) Representative images show Safranin O-Fast Green staining and BMP5 immunohistochemical staining in the knee articular cartilage sections from sham, DMM-2W and DMM-4W model mice (n=5 each). Scale bars: 10 μm (first line) and 5 μm (others). (E) Representative western blots show the levels of BMP5 protein in the knee articular cartilage sections from sham, DMM-2W and DMM-4W model mice. (F) ELISA assay results show joint fluid BMP5 levels in the healthy and OA patients. (G) BMP5 immunohistochemical staining scores and OARSI scores of the DMM-2W, DMM-4W, and sham model mice are shown. All data are represented as means ± SD.