Research Paper Volume 13, Issue 7 pp 9646—9664

BMP5 silencing inhibits chondrocyte senescence and apoptosis as well as osteoarthritis progression in mice

Deletion of BMP5 attenuates osteoarthritis in the in vivo DMM model mice. (A) Representative images show hematoxylin and eosin (H&E) staining, toluidine staining and Safranin O-Fast Green staining of knee cartilage tissue sections from LV-siBMP5 and LV-siNC group mice (n=5 per group). (B) RT-PCR results show BMP5 mRNA levels in LV-siBMP5 and LV-siNC group mice at 2 weeks post-DMM surgery (n = 5 per group). (C) Representative images show synovitis and SBP thickness in the sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. (D) OARSI scores and hyaline cartilage/calcified cartilage ratios in sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. (E) Quantitative analysis shows the status of synovitis and SBP thickness in the sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. All data are represented as the means ± SD (n=5 per group); scale bars: 5 μm.

Figure 2. Deletion of BMP5 attenuates osteoarthritis in the in vivo DMM model mice. (A) Representative images show hematoxylin and eosin (H&E) staining, toluidine staining and Safranin O-Fast Green staining of knee cartilage tissue sections from LV-siBMP5 and LV-siNC group mice (n=5 per group). (B) RT-PCR results show BMP5 mRNA levels in LV-siBMP5 and LV-siNC group mice at 2 weeks post-DMM surgery (n = 5 per group). (C) Representative images show synovitis and SBP thickness in the sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. (D) OARSI scores and hyaline cartilage/calcified cartilage ratios in sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. (E) Quantitative analysis shows the status of synovitis and SBP thickness in the sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. All data are represented as the means ± SD (n=5 per group); scale bars: 5 μm.