Figure 4. BMP5 knockdown inhibits in vitro and in vivo chondrocyte senescence in the in vitro and in vivo OA models. (A) The SA-β gal staining assay results in IL-1β-treated control and BMP5 knockdown murine chondrocytes. (B) Representative western blots show the levels of HMGB1, p53, p16, and p21 proteins in IL-1β-treated control and BMP5 knockdown murine chondrocytes. (C) Representative immunofluorescence images show γH2AX (red) staining in control and BMP5 knockdown murine chondrocytes treated with IL-1β. The chondrocytes were counterstained with DAPI (blue), a DNA binding dye. (D) Quantitative analyses show SA-β gal staining assay and γH2AX (red) staining in treated as above. (E, F) Representative immunohistochemical staining and quantitative analysis of p16 and p21 expression in the knee articular cartilage tissues from sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. All data are represented as the means ± SD (n=5); scale bars: 5 μm; **P<0.01; ***P<0.001.