Research Paper Volume 13, Issue 7 pp 9646—9664

BMP5 silencing inhibits chondrocyte senescence and apoptosis as well as osteoarthritis progression in mice

BMP5 silencing protects against chondrocyte apoptosis in both in vivo and in vitro OA models.  (A) Representative images show TUNEL staining assay in control and BMP5 knockdown murine chondrocytes treated with IL-1β. Scale bar: 5μm. (B) Quantitative analysis shows the total numbers of TUNEL-positive control and BMP5 knockdown chondrocytes treated with IL-1β. (C) Representative western blot images show BCL2 and cleaved-caspase3 protein levels in control and BMP5 knockdown chondrocytes treated with IL-1β. (D) Representative images and (E) quantitative analyses show TUNEL staining and BCl-2 immunohistochemical staining results in the knee articular cartilage tissues from sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. Scale bar: 5μm. All data are represented as the means ± SD (n=5); Scale bars: 5μm.

Figure 5. BMP5 silencing protects against chondrocyte apoptosis in both in vivo and in vitro OA models. (A) Representative images show TUNEL staining assay in control and BMP5 knockdown murine chondrocytes treated with IL-1β. Scale bar: 5μm. (B) Quantitative analysis shows the total numbers of TUNEL-positive control and BMP5 knockdown chondrocytes treated with IL-1β. (C) Representative western blot images show BCL2 and cleaved-caspase3 protein levels in control and BMP5 knockdown chondrocytes treated with IL-1β. (D) Representative images and (E) quantitative analyses show TUNEL staining and BCl-2 immunohistochemical staining results in the knee articular cartilage tissues from sham-operated, DMM plus LV-siNC, and DMM plus LV-siBMP5 groups of mice at 4 weeks post-DMM operation. Scale bar: 5μm. All data are represented as the means ± SD (n=5); Scale bars: 5μm.