Research Paper Volume 13, Issue 6 pp 8960—8974

Exercise in mice ameliorates high-fat diet-induced nonalcoholic fatty liver disease by lowering HMGCS2

HMGCS2 regulated HepG2 apoptosis in lipotoxic conditions. Cell apoptosis was assessed by TUNEL staining (green), and the nucleus was stained using propidium iodide (PI) (blue). (A) Representative images of TUNEL staining. (B) Quantification of TUNEL-positive cells from three different independent cultures. (C) Representative western blot for cleaved caspase-3 and total caspase-3. (D) Quantification of cleaved caspase-3 protein levels relative to total caspase-3 from three different cultures. Data are presented as mean ± SD, n = 3. * P P

Figure 8. HMGCS2 regulated HepG2 apoptosis in lipotoxic conditions. Cell apoptosis was assessed by TUNEL staining (green), and the nucleus was stained using propidium iodide (PI) (blue). (A) Representative images of TUNEL staining. (B) Quantification of TUNEL-positive cells from three different independent cultures. (C) Representative western blot for cleaved caspase-3 and total caspase-3. (D) Quantification of cleaved caspase-3 protein levels relative to total caspase-3 from three different cultures. Data are presented as mean ± SD, n = 3. * P < 0.05 vs control + control siRNA and † P < 0.05 vs palmitate + control siRNA.