Figure 8. HMGCS2 regulated HepG2 apoptosis in lipotoxic conditions. Cell apoptosis was assessed by TUNEL staining (green), and the nucleus was stained using propidium iodide (PI) (blue). (A) Representative images of TUNEL staining. (B) Quantification of TUNEL-positive cells from three different independent cultures. (C) Representative western blot for cleaved caspase-3 and total caspase-3. (D) Quantification of cleaved caspase-3 protein levels relative to total caspase-3 from three different cultures. Data are presented as mean ± SD, n = 3. * P < 0.05 vs control + control siRNA and † P < 0.05 vs palmitate + control siRNA.