Research Paper Volume 13, Issue 7 pp 9348—9372

miR-30c-1 encourages human corneal endothelial cells to regenerate through ameliorating senescence

miR-30c-1 ameliorates TGF-β1-induced cell cycle arrest. (A) Cell cycle analysis was analyzed using DNA content. (B) The percentage of cells in S-phase. (C) The percentage of cells in G0/G1 phase. (D) The percentage of cells in G2/M phase. (E) Representative images of cell shape. (F) Cell size was increased by TGF-β1 treatment, which was ameliorated by miR-30c-1. (G) Representative images of yes-associated protein 1 (YAP). (H) YAP levels were quantified. (I) miR-30c-l levels were reduced in treatment with TGF-β1. *statistically significant.

Figure 4. miR-30c-1 ameliorates TGF-β1-induced cell cycle arrest. (A) Cell cycle analysis was analyzed using DNA content. (B) The percentage of cells in S-phase. (C) The percentage of cells in G0/G1 phase. (D) The percentage of cells in G2/M phase. (E) Representative images of cell shape. (F) Cell size was increased by TGF-β1 treatment, which was ameliorated by miR-30c-1. (G) Representative images of yes-associated protein 1 (YAP). (H) YAP levels were quantified. (I) miR-30c-l levels were reduced in treatment with TGF-β1. *statistically significant.