Figure 7. The regulation of mTOR on autophagy in MIRI. (A) Western blot was used to detect the expression level of Beclin1, LC-3, P62, total m-TOR and p-mTOR protein. GAPDH serves as a loading control. (B) Expression of Beclin1, LC-3, P62 and p-mTOR protein relative to GAPDH data from 3 biological repeats is shown. Data shown are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. N=3 per group. Model, in vitro oxidative stress model; NC, negative control of RNAi; si, RNAi knockdown of Uhrf1; Uhrf1, Uhrf1 overexpression.