Research Paper Volume 13, Issue 6 pp 8078—8094

Flotillin-2 promotes cell proliferation via activating the c-Myc/BCAT1 axis by suppressing miR-33b-5p in nasopharyngeal carcinoma

FLOT2 promotes proliferation via positively regulating BCAT1 in NPC. (A) Western blot assay indicating the level of FLOT2 and BCAT1 in 5-8F-shFLOT2, 5-8F-shFLOT2+BCAT1, 6-10B-FLOT2, 6-10B-FLOT2+shBCAT1, and control cells. (B–D) CCK-8, plate clone formation, and EdU assays showing the growth and proliferation abilities of 5-8F-shFLOT2, 5-8F-shFLOT2+BCAT1, 6-10B-FLOT2, 6-10B-FLOT2+shBCAT1, and control cells in vitro. 5-8F-shFLOT2, 5-8F-shFLOT2+BCAT1, 6-10B-FLOT2, 6-10B-FLOT2+shBCAT1, and control cells were subcutaneously implanted into nude mice (n = 5 each group) for xenografts formation to analyze the proliferation in vivo. The pictures (E), the volumes (F), and weights (G) of xenografts were presented. *, P P P

Figure 2. FLOT2 promotes proliferation via positively regulating BCAT1 in NPC. (A) Western blot assay indicating the level of FLOT2 and BCAT1 in 5-8F-shFLOT2, 5-8F-shFLOT2+BCAT1, 6-10B-FLOT2, 6-10B-FLOT2+shBCAT1, and control cells. (BD) CCK-8, plate clone formation, and EdU assays showing the growth and proliferation abilities of 5-8F-shFLOT2, 5-8F-shFLOT2+BCAT1, 6-10B-FLOT2, 6-10B-FLOT2+shBCAT1, and control cells in vitro. 5-8F-shFLOT2, 5-8F-shFLOT2+BCAT1, 6-10B-FLOT2, 6-10B-FLOT2+shBCAT1, and control cells were subcutaneously implanted into nude mice (n = 5 each group) for xenografts formation to analyze the proliferation in vivo. The pictures (E), the volumes (F), and weights (G) of xenografts were presented. *, P <0.05, **, P < 0.01, ***; P < 0.001.