Figure 3. GABA antagonizes the pro-proliferative effects of BCAT1 in NPC. (A) CCK-8 assay indicating the effects of GABA at different concentration on 5-8F and 6-10B cells. (B, C) plate clone formation and EdU assays showing the inhibitory effects of GABA at 20mM on proliferation of 5-8F cells. (D) Western blot demonstrating the levels of BCAT1 and FLOT2 in 5-8F cells treated by GABA at 20mM. (E–G) CCK-8, plate clone formation, and EdU assays showing inhibitory effects on 6-10B-FLOT2 cells treated by GABA at 20mM. **, P < 0.01, ***; P < 0.001.