Research Paper Volume 13, Issue 6 pp 8078—8094

Flotillin-2 promotes cell proliferation via activating the c-Myc/BCAT1 axis by suppressing miR-33b-5p in nasopharyngeal carcinoma

FLOT2 regulates c-Myc and promotes cell proliferation via inhibiting miR-33b-5p in NPC. (A) qPCR assay demonstrating the relative expression of miR-33b-5p in 5-8F-shFLOT2, 6-10B-BCAT1 and control cells. (B) Western blot showing the level of c-Myc in 5-8F and 6-10B cells transfected with miR-33b-5p mimic and inhibitor, respectively. (C) Luciferase activities of 5-8F cells co-transfected with miR-33b-5p+wild type c-Myc and miR-33b-5p+mutant type c-Myc, respectively. (D) Western blot showing the level of c-Myc in 5-8F-shFLOT2, 5-8F-shFLOT2+miR-33b-5p inhibitor, 5-8F-shFLOT2+miR-33b-5p inhibitor+sic-Myc, 6-10B-FLOT2, 6-10B-FLOT2+miR-33b-5p mimic, 6-10B-FLOT2+miR-33b-5p mimic+c-Myc and control cells. (E, F) Plate clone formation and EdU assays showing the growth and proliferation abilities of 5-8F-shFLOT2, 5-8F-shFLOT2+miR-33b-5p inhibitor, 5-8F-shFLOT2+miR-33b-5p inhibitor+sic-Myc, 6-10B-FLOT2, 6-10B-FLOT2+miR-33b-5p mimic, 6-10B-FLOT2+miR-33b-5p mimic+c-Myc cells. *, P P P

Figure 5. FLOT2 regulates c-Myc and promotes cell proliferation via inhibiting miR-33b-5p in NPC. (A) qPCR assay demonstrating the relative expression of miR-33b-5p in 5-8F-shFLOT2, 6-10B-BCAT1 and control cells. (B) Western blot showing the level of c-Myc in 5-8F and 6-10B cells transfected with miR-33b-5p mimic and inhibitor, respectively. (C) Luciferase activities of 5-8F cells co-transfected with miR-33b-5p+wild type c-Myc and miR-33b-5p+mutant type c-Myc, respectively. (D) Western blot showing the level of c-Myc in 5-8F-shFLOT2, 5-8F-shFLOT2+miR-33b-5p inhibitor, 5-8F-shFLOT2+miR-33b-5p inhibitor+sic-Myc, 6-10B-FLOT2, 6-10B-FLOT2+miR-33b-5p mimic, 6-10B-FLOT2+miR-33b-5p mimic+c-Myc and control cells. (E, F) Plate clone formation and EdU assays showing the growth and proliferation abilities of 5-8F-shFLOT2, 5-8F-shFLOT2+miR-33b-5p inhibitor, 5-8F-shFLOT2+miR-33b-5p inhibitor+sic-Myc, 6-10B-FLOT2, 6-10B-FLOT2+miR-33b-5p mimic, 6-10B-FLOT2+miR-33b-5p mimic+c-Myc cells. *, P <0.05, **, P < 0.01, ***; P < 0.001.