Research Paper Volume 13, Issue 7 pp 9748—9765

Long non-coding RNA DPP10-AS1 exerts anti-tumor effects on colon cancer via the upregulation of ADCY1 by regulating microRNA-127-3p

Prediction and verification of the binding relationship among DPP10-AS1, ADCY1, and miR-127-3p. (A) the heat map of microarray data GSE41328. (B) the expression of DPP10-AS1 in colon cancer tissues and adjacent normal tissues in TCGA database. * p  0.05 compared with adjacent normal tissues. (C) the location of DPP10-AS1 in cells by website prediction and FISH assay. (D) the expression of DPP10-AS1, miR-127-3p, and ADCY1 in cancer and adjacent normal tissues detected by RT-qPCR. * p E) Pearson’s correlation coefficient on the correlation among DPP10-AS1, miR-127-3p, and ADCY1 in colon cancer. (F) binding relation between DPP10-AS1 and miR-127-3p predicted on a bioinformatics website and dual luciferase reporter gene assay. * p G) the binding site between miR-127-3p and ADCY1 predicted using bioinformatics analysis and dual luciferase reporter gene assay. * p H) interaction between DPP10-AS1 and miR-127-3p verified by RIP assay. * p  0.05 compared with IgG. (I) enrichment of miR-127-3p in DPP10-AS1 revealed by RNA pull-down assay. *, p vs. DPP10-AS1 NC. Measurement data were expressed as mean ± standard deviation. The data were analyzed by t-test. Data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The experiment was repeated three times.

Figure 1. Prediction and verification of the binding relationship among DPP10-AS1, ADCY1, and miR-127-3p. (A) the heat map of microarray data GSE41328. (B) the expression of DPP10-AS1 in colon cancer tissues and adjacent normal tissues in TCGA database. * p < 0.05 compared with adjacent normal tissues. (C) the location of DPP10-AS1 in cells by website prediction and FISH assay. (D) the expression of DPP10-AS1, miR-127-3p, and ADCY1 in cancer and adjacent normal tissues detected by RT-qPCR. * p < 0.05 compared with adjacent normal tissues. (E) Pearson’s correlation coefficient on the correlation among DPP10-AS1, miR-127-3p, and ADCY1 in colon cancer. (F) binding relation between DPP10-AS1 and miR-127-3p predicted on a bioinformatics website and dual luciferase reporter gene assay. * p < 0.05 compared with NC mimic treatment. (G) the binding site between miR-127-3p and ADCY1 predicted using bioinformatics analysis and dual luciferase reporter gene assay. * p < 0.05 compared with NC mimic treatment. (H) interaction between DPP10-AS1 and miR-127-3p verified by RIP assay. * p < 0.05 compared with IgG. (I) enrichment of miR-127-3p in DPP10-AS1 revealed by RNA pull-down assay. *, p < 0.05 vs. DPP10-AS1 NC. Measurement data were expressed as mean ± standard deviation. The data were analyzed by t-test. Data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The experiment was repeated three times.