Research Paper Volume 13, Issue 7 pp 9748—9765

Long non-coding RNA DPP10-AS1 exerts anti-tumor effects on colon cancer via the upregulation of ADCY1 by regulating microRNA-127-3p

HT-29 and SW480 cells are successfully sorted out. (A) expression of stem cell markers detected by RT-qPCR. (B) expression of DPP10-AS1, miR-127-3p, and ADCY1 determined using RT-qPCR. Measurement data were expressed as mean ± standard deviation. The data between two groups were analyzed by unpaired t-test. Data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The experiment was repeated three times. * p

Figure 2. HT-29 and SW480 cells are successfully sorted out. (A) expression of stem cell markers detected by RT-qPCR. (B) expression of DPP10-AS1, miR-127-3p, and ADCY1 determined using RT-qPCR. Measurement data were expressed as mean ± standard deviation. The data between two groups were analyzed by unpaired t-test. Data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The experiment was repeated three times. * p < 0.05 compared with CD133 negative cells.