Research Paper Volume 13, Issue 7 pp 9748—9765

Long non-coding RNA DPP10-AS1 exerts anti-tumor effects on colon cancer via the upregulation of ADCY1 by regulating microRNA-127-3p

Upregulation of DPP10-AS1 or downregulation of miR-127-3p elevates the ADCY1 protein expression and diminishes the protein expression of CD44, Lgr5, and ALDH1. (A–C) the mRNA expression, the immunoblots and protein expression of stem cell genes determined using RT-qPCR and western blot analysis respectively after DPP10-AS1 expression was altered. (D–F) the mRNA expression, the immunoblots and protein expression of stem cell genes evaluated using RT-qPCR and western blot analysis respectively after miR-127-3p expression was altered. *, p vs. vector and si-NC or NC mimic and NC inhibitor. The measurement data were expressed as mean ± standard deviation. The data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The experiment was repeated three times. DPP10-AS1, cells transfected with DPP10-AS1 plasmid; si-DPP10-AS1, cells transfected with si-DPP10-AS1; si-NC, cells transfected with si-negative control plasmid; miR-127-3p mimic, cells transfected with miR-127-3p mimic plasmid; NC mimic, cells transfected with negative control mimic plasmid.

Figure 3. Upregulation of DPP10-AS1 or downregulation of miR-127-3p elevates the ADCY1 protein expression and diminishes the protein expression of CD44, Lgr5, and ALDH1. (AC) the mRNA expression, the immunoblots and protein expression of stem cell genes determined using RT-qPCR and western blot analysis respectively after DPP10-AS1 expression was altered. (DF) the mRNA expression, the immunoblots and protein expression of stem cell genes evaluated using RT-qPCR and western blot analysis respectively after miR-127-3p expression was altered. *, p < 0.05 vs. vector and si-NC or NC mimic and NC inhibitor. The measurement data were expressed as mean ± standard deviation. The data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The experiment was repeated three times. DPP10-AS1, cells transfected with DPP10-AS1 plasmid; si-DPP10-AS1, cells transfected with si-DPP10-AS1; si-NC, cells transfected with si-negative control plasmid; miR-127-3p mimic, cells transfected with miR-127-3p mimic plasmid; NC mimic, cells transfected with negative control mimic plasmid.