Research Paper Volume 13, Issue 7 pp 9748—9765

Long non-coding RNA DPP10-AS1 exerts anti-tumor effects on colon cancer via the upregulation of ADCY1 by regulating microRNA-127-3p

Upregulation of DPP10-AS1 or downregulation of miR-127-3p inhibits tumor growth in HT-29 and SW480 stem cells transplanted into nude mice. (A–C) the tumor growth and size after the expression of DPP10-AS1 was intervened. (D–F) the tumor growth and size after the expression of miR-127-3p was altered. *, p vs. vector and si-NC, or NC mimic and anta-Ctrl. The measurement data were expressed as mean ± standard deviation. The data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The data at different time points were analyzed by repeated measures ANOVA followed by Bonferroni’s post hoc test for n = 5. The experiment was repeated three times. DPP10-AS1, cells transfected with DPP10-AS1 plasmid; si-DPP10-AS1, cells transfected with si-DPP10-AS1; si-NC, cells transfected with si-negative control plasmid; miR-127-3p mimic, cells transfected with miR-127-3p mimic plasmid; anta-miR-127-3p, cells transfected with anta-miR-127-3p plasmid; NC mimic, cells transfected with negative control mimic plasmid; anta-Ctrl, cells transfected with anta-Ctrl plasmid.

Figure 7. Upregulation of DPP10-AS1 or downregulation of miR-127-3p inhibits tumor growth in HT-29 and SW480 stem cells transplanted into nude mice. (AC) the tumor growth and size after the expression of DPP10-AS1 was intervened. (DF) the tumor growth and size after the expression of miR-127-3p was altered. *, p < 0.05 vs. vector and si-NC, or NC mimic and anta-Ctrl. The measurement data were expressed as mean ± standard deviation. The data among multiple groups were analyzed by one-way ANOVA followed by a Tukey’s post hoc test. The data at different time points were analyzed by repeated measures ANOVA followed by Bonferroni’s post hoc test for n = 5. The experiment was repeated three times. DPP10-AS1, cells transfected with DPP10-AS1 plasmid; si-DPP10-AS1, cells transfected with si-DPP10-AS1; si-NC, cells transfected with si-negative control plasmid; miR-127-3p mimic, cells transfected with miR-127-3p mimic plasmid; anta-miR-127-3p, cells transfected with anta-miR-127-3p plasmid; NC mimic, cells transfected with negative control mimic plasmid; anta-Ctrl, cells transfected with anta-Ctrl plasmid.