Research Paper Volume 13, Issue 7 pp 9780—9800

Lung cancer-associated mesenchymal stem cells promote tumor metastasis and tumorigenesis by induction of epithelial–mesenchymal transition and stem-like reprogram

Tumor invasion was induced by LC-MSCs in vitro. A549.CopGFP and H1299.CopGFP cells were transduced by lentiviral particles to express CopGFP stably firstly. Then the invasion ability of these cells was tested by transwell invasion assay after they were co-incubated with LC-MSCs or not for 24 hours. (A, B) A549 cells were co-incubated with LC-MSCs at the cell ratio 1:1 and 1:10. (C, D) H1299 cells were co-incubated with LC-MSCs at the cell ratio 1:1 and 1:10. Color bar chart, triplicate data for each patient. Dark bar chart, statistical analysis for all patients. Fluorescent pictures illustrated tumor cells that had migrated through the membrane of transwell inserts. TF-MSCs, tumor-free mesenchymal stem cells, were used as comparison from normal lung tissues. **, P

Figure 2. Tumor invasion was induced by LC-MSCs in vitro. A549.CopGFP and H1299.CopGFP cells were transduced by lentiviral particles to express CopGFP stably firstly. Then the invasion ability of these cells was tested by transwell invasion assay after they were co-incubated with LC-MSCs or not for 24 hours. (A, B) A549 cells were co-incubated with LC-MSCs at the cell ratio 1:1 and 1:10. (C, D) H1299 cells were co-incubated with LC-MSCs at the cell ratio 1:1 and 1:10. Color bar chart, triplicate data for each patient. Dark bar chart, statistical analysis for all patients. Fluorescent pictures illustrated tumor cells that had migrated through the membrane of transwell inserts. TF-MSCs, tumor-free mesenchymal stem cells, were used as comparison from normal lung tissues. **, P < 0.05 were considered to be statistically significant.