Research Paper Volume 13, Issue 7 pp 9780—9800

Lung cancer-associated mesenchymal stem cells promote tumor metastasis and tumorigenesis by induction of epithelial–mesenchymal transition and stem-like reprogram

Tumor migration influenced by LC-MSCs in vitro. (A–D) A549.CopGFP and H1299.CopGFP cells were co-incubated with LC-MSCs at the cell ratio 1:1 and 1:10 to 80% confluence before wound healing assay was performed. The migrated ratio was calculated as (the first scratch distance - the scratch distance 48 hours later)/ the first scratch distance×100%. Color bar chart, triplicate data for each patient. Dark bar chart, statistical analysis for all patients. TF-MSCs, comparison from normal lung tissues. (E) The migratory capacity of A549 cells in response to conditioned medium of LC-MSCs was determined using transwell migration assay. Control, serum-free medium without LC/TF-MSCs. *, P

Figure 3. Tumor migration influenced by LC-MSCs in vitro. (AD) A549.CopGFP and H1299.CopGFP cells were co-incubated with LC-MSCs at the cell ratio 1:1 and 1:10 to 80% confluence before wound healing assay was performed. The migrated ratio was calculated as (the first scratch distance - the scratch distance 48 hours later)/ the first scratch distance×100%. Color bar chart, triplicate data for each patient. Dark bar chart, statistical analysis for all patients. TF-MSCs, comparison from normal lung tissues. (E) The migratory capacity of A549 cells in response to conditioned medium of LC-MSCs was determined using transwell migration assay. Control, serum-free medium without LC/TF-MSCs. *, P < 0.05 were considered to be statistically significant.