Research Paper Volume 13, Issue 7 pp 9780—9800

Lung cancer-associated mesenchymal stem cells promote tumor metastasis and tumorigenesis by induction of epithelial–mesenchymal transition and stem-like reprogram

EMT-associated gene expression induced by LC-MSCs. (A) Equal number of A549.CopGFP and LC-MSCs were co-cultured directly for 48 hours and then sorted by cytometry flow to test the expression EMT-associated genes by realtime PCR. (B, C) Equal number of A549.CopGFP and LC-MSCs were co-incubated separately by transwell inserts for 48 hours. The EMT-associated gene expression in A549 cells was evaluated by realtime PCR (B) and western blot (C), individually. Representative results of three patients. TF-MSCs, comparison from normal lung tissues. (D), The level of TGF-β secreted by MSCs that derived from different tissues was tested by ELISA assay. (E), The induced snail and slug expression was blocked by TGF-β signaling inhibitor galunisertib. A549 cells were pre-incubated with galunisertib (5 μM) for 30 min, then treated by the supernatants of LC/NC-MSCs from 3 patients. *, P

Figure 4. EMT-associated gene expression induced by LC-MSCs. (A) Equal number of A549.CopGFP and LC-MSCs were co-cultured directly for 48 hours and then sorted by cytometry flow to test the expression EMT-associated genes by realtime PCR. (B, C) Equal number of A549.CopGFP and LC-MSCs were co-incubated separately by transwell inserts for 48 hours. The EMT-associated gene expression in A549 cells was evaluated by realtime PCR (B) and western blot (C), individually. Representative results of three patients. TF-MSCs, comparison from normal lung tissues. (D), The level of TGF-β secreted by MSCs that derived from different tissues was tested by ELISA assay. (E), The induced snail and slug expression was blocked by TGF-β signaling inhibitor galunisertib. A549 cells were pre-incubated with galunisertib (5 μM) for 30 min, then treated by the supernatants of LC/NC-MSCs from 3 patients. *, P < 0.05 were considered to be statistically significant. P1-3, three patients. 1, Tumor cells alone. 2, Tumor cells + LC-MSCs. 2, Tumor cells + TF-MSCs. Data from triplicate experiments.