Research Paper Volume 13, Issue 7 pp 9780—9800

Lung cancer-associated mesenchymal stem cells promote tumor metastasis and tumorigenesis by induction of epithelial–mesenchymal transition and stem-like reprogram

Stem-like characteristics induced by LC-MSCs. (A) Equal number of A549.CopGFP and LC-MSCs were co-cultured directly for 48 hours and then sorted by cytometry flow to test the stem-related gene expression by realtime PCR. (B) Equal number of A549.CopGFP and LC-MSCs were co-incubated separately by transwell inserts for 48 hours. The expression of stem-related genes in A549 cells was evaluated by realtime PCR. (C) Different cell ratio of A549.CopGFP and LC-MSCs. DsRed were co-incubated in 96-well 3D cell culture plates. The spheroid formation was observed under inverted fluorescence microscope on the second day of plating cells. (D) Spheroid grew observed at indicated time. *, P

Figure 5. Stem-like characteristics induced by LC-MSCs. (A) Equal number of A549.CopGFP and LC-MSCs were co-cultured directly for 48 hours and then sorted by cytometry flow to test the stem-related gene expression by realtime PCR. (B) Equal number of A549.CopGFP and LC-MSCs were co-incubated separately by transwell inserts for 48 hours. The expression of stem-related genes in A549 cells was evaluated by realtime PCR. (C) Different cell ratio of A549.CopGFP and LC-MSCs. DsRed were co-incubated in 96-well 3D cell culture plates. The spheroid formation was observed under inverted fluorescence microscope on the second day of plating cells. (D) Spheroid grew observed at indicated time. *, P < 0.05 were considered to be statistically significant. P1-3, three patients. Data from triplicate experiments.