Research Paper Volume 13, Issue 7 pp 9820—9837

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy

Upregulation of HDAC6 in mouse knees and chondrocytes. (A, B) Typical Safranin O staining of the cartilage and subchondral cortical bone in each group of mice, scale bar = 200 μm, scale bar (enlarged) = 50 μm. (C, D) IF staining and quantification of the changes in HDAC6 in control and OA mice, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (E, F) Western blotting and quantification of HDAC6 in chondrocytes subjected to different concentrations of TBHP for 12 hours. (G, H) Western blotting and quantification of HDAC6 in chondrocytes subjected to 50 μM of TBHP in a time-dependent manner. N = 5, GAPDH was the loading control, significance: *P

Figure 1. Upregulation of HDAC6 in mouse knees and chondrocytes. (A, B) Typical Safranin O staining of the cartilage and subchondral cortical bone in each group of mice, scale bar = 200 μm, scale bar (enlarged) = 50 μm. (C, D) IF staining and quantification of the changes in HDAC6 in control and OA mice, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (E, F) Western blotting and quantification of HDAC6 in chondrocytes subjected to different concentrations of TBHP for 12 hours. (G, H) Western blotting and quantification of HDAC6 in chondrocytes subjected to 50 μM of TBHP in a time-dependent manner. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.