Research Paper Volume 13, Issue 7 pp 9838—9858

lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis

Effects of SNHG4 on CRC cell proliferation and metastasis. (A) SNHG4 was knocked down in HCT116 and SW620 cells by transfection of the cells with si-SNHG4 1/2/3. The transfection efficiency was validated by real-time PCR. Si-SNHG4 2 was selected for further experiments due to its better transfection efficiency. (B–C) HCT116 and SW620 cells were transfected with si-SNHG4, and (B) cell viability was examined by CCK-8 assay and (C) colony formation capacity was examined by colony formation assay. (D) HCT116 and SW620 cells were transfected with si-SNHG4, and cell invasion ability was examined by Transwell assay. *P **P

Figure 2. Effects of SNHG4 on CRC cell proliferation and metastasis. (A) SNHG4 was knocked down in HCT116 and SW620 cells by transfection of the cells with si-SNHG4 1/2/3. The transfection efficiency was validated by real-time PCR. Si-SNHG4 2 was selected for further experiments due to its better transfection efficiency. (B–C) HCT116 and SW620 cells were transfected with si-SNHG4, and (B) cell viability was examined by CCK-8 assay and (C) colony formation capacity was examined by colony formation assay. (D) HCT116 and SW620 cells were transfected with si-SNHG4, and cell invasion ability was examined by Transwell assay. *P < 0.05, **P < 0.01, compared to the si-NC group.